Bono, The Edge, Adam Clayton and Larry Mullen were announced as performers via MTV EMAs official Twitter account today.
The group released their 13th studio album Songs of Innocence via iTunes back in September.
The release made the album available to over 500 million iTunes customers for free.
This will be the fourth time U2 have been part of the MTV EMA, having performed in Rotterdam in 1997, Stockholm in 2000 and from Berlin's Brandenburg Gate in 2009.
On Sunday hip hop superstar Nicki Minaj the award show and also perform on the night joining a cast of other confirmed performers including Enrique Iglesias, Calvin Harris, Ed Sheeran, Ariana Grande, Charli XCX, Kiesza and Royal Blood.
Rock legend Ozzy Osbourne will also be honoured with the Global Icon Award.
Councillor Gordon Matheson, Leader of Glasgow City Council and Chair of Glasgow City Marketing Bureau, said: “Today’s exciting announcement that U2 – one of the biggest names in music – is to join the incredible list of acts already confirmed to perform at the MTV EMA this weekend will come as welcome news to music fans across the city. In a little over 48 hours’ time, this year’s EMA will showcase Glasgow to more than half a billion households through MTV’s global network of channels, reinforcing our enviable reputation as a world-class music destination.”